Antioxidant capacity, reproductive response and glucose concentration and insulin of ewes supplemented prior to insemination with orange residue / [Capacidade antioxidante, resposta reprodutiva e concentração de glicose e insulina de ovelhas suplementadas antes da inseminação com resíduo de laranja]

The objective was to evaluate the antioxidant capacity, glucose and insulin concentration and reproductive performance of ewes supplemented with orange residue prior to insemination. Fifty-five multiparous ewes were divided into two corrals, and 15 unbred ewes were kept individually to measure feed consumption. Two integral diets were administered; T0: control treatment and T1: with 20% of dry matter of orange residue. Ten days after the start of supplementation, the ewes were synchronized. Supplementation was finalized prior to artificial insemination, then, a blood sample was taken to measure the antioxidant capacity and glucose and insulin concentration. An analysis of variance was made to evaluate the effect of treatment on the antioxidant capacity, glucose and insulin; and to analyze the response to estrus, percentage of gestation and prolificity a ji squared test was performed. Of 9 antioxidant compounds found in the orange residue, hesperidin (7.44%), chlorogenic acid (0.50%) and protocatechuic acid had the highest concentration. Feed intake, estrus response, percentage of gestation, antioxidant capacity, and glucose and insulin concentration were not affected by the treatment. It is concluded that inclusion of 20% of orange residue in the diet prior to insemination in ewes is possible.(AU)

O objetivo deste estudo foi avaliar a capacidade antioxidante, a concentração de glicose e insulina e o comportamento reprodutivo de ovelhas suplementadas com resíduo de laranja antes da inseminação. Cinquenta e cinco ovelhas multíparas foram divididas em dois currais e 15 ovelhas sem raça foram mantidas individualmente para se medir o consumo de ração. Duas dietas integrais foram administradas; T0: tratamento controle e T1: dieta com 20% de resíduo de laranja seco. Dias após o início da suplementação, as ovelhas foram sincronizadas. A suplementação foi finalizada antes da inseminação artificial e, em seguida, foi coletada uma amostra de sangue para medir a capacidade antioxidante e a concentração de glicose e insulina. Uma análise de variância foi feita para avaliar o efeito do tratamento sobre a capacidade antioxidante, a glicose e a insulina, e um teste do ji quadrado foi realizado para analisar a resposta ao estro, a porcentagem de gestação e de prolificidade. Dos nove compostos antioxidantes encontrados no resíduo laranja, a hesperidina (7,44%), o ácido clorogênico (0,50%) e o ácido protocatecuico foram os de maior concentração. O consumo alimentar, a resposta ao estro, a porcentagem de gestação, a capacidade antioxidante, a concentração de glicose e a insulina não foram afetados pelo tratamento. Conclui-se que é possível a inclusão de 20% de resíduos de laranja na dieta antes da inseminação em ovelhas.(AU)

Farmacopuntura utilizando os acupontos Hou Hai e Bai Hui em protocolos de sincronização de estro em cabras / Pharmacopuncture using acupoints Hou Hai and Bai Hui in estrous synchronization protocols in goats

Avaliou-se a eficiência da administração de subdoses de eCG nos acupontos Bai Hui e Hou Hai em protocolos de sincronização de estro em cabras. Na primeira etapa, 57 cabras foram distribuídas aleatoriamente em quatro tratamentos: T1- 300UI de eCG intramuscular (IM); T2- 60UI de eCG no acuponto Hou Hai; T3- 60UI de eCG no acuponto Bai Hui e T4- 60UI de eCG IM; e na segunda etapa, 28 cabras foram distribuídas aleatoriamente em três tratamentos: T1- 300UI de eCG IM; T2- 30UI de eCG no acuponto Bai Hui e T3- 30UI de eCG IM. Ao final do tratamento hormonal, as cabras foram monitoradas para detecção do estro, realização das coberturas e avaliação do comportamento reprodutivo. Os dados foram submetidos à análise de normalidade, seguida dos testes estatísticos adequados para cada variável. Na primeira etapa experimental, obteve-se maior duração de estro nas cabras do T1 (P=0,009). Na segunda etapa experimental, obteve-se maior número de animais em estros no T1 (P=0,03). As demais variáveis para ambas as etapas não sofreram influência dos tratamentos (P>0,05), demonstrando que a administração de subdoses de eCG nos acupontos Bai Hui e Hou Hai foi eficiente para sincronizar o estro.(AU)

The efficiency of administration of subdoses of eCG in the Bai Hui and Hou Hai acupoints in oestrus synchronization protocols in goats was evaluated. In the first stage, 57 goats were randomly assigned to four treatments: T1- 300UI of intramuscular eCG (IM); T2- 60UI of eCG in acupoint Hou Hai; T3- 60UI of eCG in the Bai Hui acupoint and T4- 60UI of eCG IM; and in the second stage, 28 goats were randomly assigned to three treatments: T1-300UI of eCG IM; T2-30UI of eCG in the Bai Hui acupoint and T3- 30UI of eCG IM. At the end of the hormonal treatment the goats were monitored for estrus detection, and evaluation of reproductive behavior. The data were submitted to normality analysis, followed by appropriate statistical tests for each variable. In the first experimental stage, a longer duration of estrus in the T1 goats (P= 0.009) was obtained. In the second experimental stage, a greater number of animals were obtained in estrus at T1 (P= 0.03). The other variables for both experiments were not influenced by the treatments (P> 0.05), demonstrating that administration of eCG subdoses in the Bai Hui and Hou Hai acupoints was efficient to synchronize the estrus.(AU)

Use of a domestic Korean black goat (Capra hircus coreanae) with its chest crayon-harnessed in detecting estrus of Himalayan tahrs (Hemitragus jemlahicus)

The reliability of a Korean black goat (Capra hircus coreanae) to detect estrus in Himalayan tahrs (Hemitragus jemlahicus) for an artificial breeding program was investigated. Estrus in six female Himalayan tahrs was synchronized using fluorogestone acetate (FGA) sponges. Thirteen days later, 200 IU of PMSG and 100 IU of hCG were injected before removing the sponges and simultaneously injecting 5 mg of PGF2alpha the next day. Penetration of the cervical canal and the thickness and location of red crayon marks were examined 40~43 h later. Two females treated with sponges containing 60 or 45 mg of FGA had estrogen levels of 8.7 and 11.1 pg/mL, respectively. No red marks were found on the backs of these two tahrs. The remaining females had higher levels of estradiol, and the red crayon marks were clearly shown. The cervical folds of these tahrs were readily penetrated and the insemination gun was smoothly inserted into the uterine body. In conclusion, a Korean domestic goat with its chest crayon-harnessed was successfully used to detect estrus of Himalayan tahrs. This technique might be utilized as a part of breeding programs for wild goats and avoid the need for a vasectomy of conspecific males.

Comparison between two progesterone sources and two oestradiol formulations in a Heatsynch protocol for postpartum cycling dairy cows in pasture

To compare an injectable progesterone (MAD-4) with an intravaginal device (IPD), and natural O17 with synthetic oestradiol (OB) in a synchronisation protocol, 51 cows were divided into four groups. Each group was treated with one of the two sources of progesterone and one of the two oestradiol formulations. Oestrus behaviour, follicle diameter, and pregnancy rates were evaluated. Oestrus behaviour (p = 0.902), numbers of cows in oestrus (p = 0.917), follicle diameter (p = 0.416), and pregnancy rates (p = 0.873) were similar among the four groups. More cows in the group treated with the IPD and OB scored > 200 oestrus behaviour points compared to the other groups (p = 0.038). A longer interval between the end of treatment and oestrus was observed among cows treated with MAD-4 than cows given the IPD (p = 0.030), but no differences were found between animals receiving the two oestradiol formulations (OB and O17). While the use of MAD-4 requires further testing, similar responses to natural oestradiol observed in the present study could allow the use of this formulation in reproductive protocols because it is not associated with the potential human health risks of OB.

Excretion of Brucella abortus vaccine B19 strain during a reproductive cycle in dairy cows

This paper aimed to determine the excretion period of B19 vaccine strain during a complete reproductive cycle (from estrus synchronization, artificial insemination, pregnancy and until 30 days after parturition) of dairy cows from 3 to 9 years old that were previously vaccinated from 3 to 8 months. Three groups were monitored with monthly milk and urine collection during 12 months: G1 with seven cows from 3 to 4 years old; G2 with three cows from 5 to 6 years old; and G3 with four cows from 7 to 9 years old. Urine and milk samples were submitted to bacteriological culture and urine and PCR reactions for detection of Brucella spp. and PCR-multiplex for B19 strain identification. Ring test (RT) was also performed in the milk samples, and serum samples were tested by buffered acidified plate antigen test (BAPA). All animals were serologically negative at BAPA and Brucella spp. was not isolated from both urine and milk samples. RT revealed 13/210 (6.2%) positive milk samples. PCR reactions detected DNA of Brucella spp. in 86/420 (20.5%) samples. In urine it was found a significantly higher frequency (35.2%; 74/210) than in milk (5.7%; 12/210), more frequently from the estrus to 150 days of pregnancy and after parturition (6.7%; 10/150), and from 150 days of pregnancy to parturition (3.4%; 2/60), and they were all identified as B19 strain. In three groups, intermittent excretion of B19 strain was detected mainly in urine samples, which confirmed its multiplication and persistence in cows for until 9 years.

Evaluación de la fertilidad de hembras bos indicus, de acuerdo a la intensidad del celo, manejadas en un programa de inseminación artificial a tiempo fijo en condiciones de trópico / Fertility assessment of bos indicus cows according to the estrus intensity, in a fixed time artificial insemination program under tropical conditions

El objetivo del presente estudio fue evaluar la fertilidad obtenida en un hato, según dos tiempos de Inseminación Artificial (IA) a tiempo fijo: 48 y 72 horas, posterior a un programa de sincronización de celos, tomando en cuenta las manifestaciones y la intensidad del estro. Se utilizaron 114 hembras Bos indicus, sincronizadas con 2mg de benzoato de estradiol más un implante intravaginal de progesterona (CIDR), que permaneció in situ por 9 días. Al retiro del CIDR, los animales fueron divididos en 2 grupos: 61 y 53 hembras, que fueron inseminadas 48 horas y 72 horas postratamiento, respectivamente. Todas fueron observadas continuamente durante 90 horas, con el fin de detectar el comportamiento sexual y clasificarlas, de acuerdo a la intensidad del celo, en dos categorías: alta y baja. Se utilizó la prueba de Ji-cuadrado para la comparación de proporciones. El porcentaje de hembras con manifestación estral fue de 68,4 por ciento, sin diferencia entre las categorías alta y baja intensidad (37,7 y 30,7 por ciento, respectivamente). El 85,4 por ciento de los animales con signos de estro ovuló, diferente de las hembras sin manifestación estral, con 63,8 por ciento (P<0,05). El porcentaje global de preñez fue 25,4 por ciento; el 86,2 por ciento de estas gestaciones corresponde al grupo de hembras que manifestaron conducta estral. De acuerdo al momento de IA y la intensidad del estro, los porcentajes de gestaciones para IA a 48 horas fueron 1) alta: 91,6 por ciento, 2) baja: 84,6 por ciento (P<0,05) y 3) sin celo: 25 por ciento, por su parte, en las hembras inseminadas a las 72 horas los porcentajes fueron: 1) alta: 8,4 por ciento, 2) baja: 15,4 por ciento y 3) sin celo: 75 por ciento (P<0,05). Se concluye que las hembras con expresión estral tienen mayor oportunidad de preñarse tras la IA a 48 horas postratamiento hormonal, mientras que en los animales sin manifestación de estros, los mayores porcentajes de gestaciones son a las 72 horas.

The aim of this study was to evaluate the fertility obtained in a herd, according to two times of fixed time AI: 48 and 72 hours, following an estrus synchronization program, taking into consideration the manifestations and the intensity of estrus. One hundred and fourteen Bos indicus females were used; synchronized with 2mg of estradiol benzoate and a progesterone-releasing intravaginal device (PRID), which remained in situ for 9 days. After the removal of the PRID, the animals were divided into two groups: 61 and 53 females that were inseminated, respectively, 48 and 72 hours posttreatment. All were continuously observed for 90 hours, with the objective of detecting sexual behavior and classifying them according to heat intensity into two categories: high and low. Chi-square test was used for the comparison of proportions. The percentage of females with estrus manifestation was 68.4 percent, without difference between high and low intensity categories (37.7 and 30.7 percent, respectively). Of the animals with signs of estrus, 85.4 percent ovulated; different from the females without estrus manifestation, with 63.8 percent (P<0.05). Global pregnancy rate was 25.4 percent; 86.2 percent of these gestations correspond to the group of females that manifested estral behavior. According to the moment of AI and the intensity of the estrus, the percentages of pregnancy for AI at 48 hours were 1) high: 91.6 percent, 2) low: 84.6 percent (P<0.05) and 3) without estrus: 25 percent, on the other hand, in females inseminated at 72 hours, the percentages were: 1) high: 8.4 percent, 2) low: 15.4 percent and 3) without estrus: 75 percent (P<0.05). It is concluded that females with estral manifestation are more likely to get pregnant after AI at 48 hours following hormonal treatment, while for animals without estral manifestation, the higher pregnancy percentages are at 72 hours.

Embryo transfer in angus and brahman recipient cows: effect of two methods of estrus synchronization on induced estrus and pregnancy

Over a 3-year period, 88 Angus and 87 Brahman cows were used as recipients to determine the effects of breedtype and method of estrous synchronization on induced estrus and subsequent pregnancy following embryo transfer. Estrus was synchronized in recipients using either prostaglandin-F2α (PGF; Lutalyse) or progestogen (PRO; Syncro-Mate-B). Recipients were treated (im) with PGF on day -11 (25 mg), 0 (12.5 mg), and 1 (12.5 mg). Recipients were treated with PRO on day -9 (6 mg norgestomet ear implant and 2 mL [im] of 3 mg of norgestomet and 5 mg of estradiol valerate) and the ear implant was removed on day 0. Embryos were randomly transferred to recipients synchronous +24 to -24 h with a 7-d embryo. Both synchronization methods (PGF vs. PRO) were similarly effective in inducing estrus (89.8 vs. 86.7 percent). Percentage of induced estrus was similar between Brahman (91.0 percent) and Angus (85.6 percent). Recipients treated with PGF had a longer (P = 0.001) interval to estrus than PRO treated recipients (77.4 vs. 60.1 h). Estrus response in Angus was earlier (P = 0.043) than in Brahman (65.4 vs. 72.2 h). Neither breed (P = 0.97; Angus 49 percent 35/72 and Brahman 54 percent 38/70) nor estrus synchronization treatment (P = 0.23; PRO 49 percent 35/72 and PGF 54 percent 38/70) affected pregnancy rate. Pregnancy rates in recipients closely synchronized (0 h) was 60.0 percent, within +12 and -12 h was 56.0 percent, and within +24 and -24 h was 51.5 percent. Four-year-old recipients had lower pregnancy rates (34.8 percent) than did 5-year-old (60.9 percent) or greater than 6-year-old (70.0 percent) recipients. These results indicate that PGF, when administered as a split-second dose, is as effective in synchronizing estrus in Angus and Brahman as PRO. Asynchrony of embryo age with recipient stage of cycle up to +24 or -24 h did not significantly affect pregnancy rates.

Durante tres años, vacas receptoras Angus (88) y Brahman (87) se utilizaron para determinar los efectos de raza y método de sincronización sobre las características del celo inducido y la preñez subsecuente a la transferencia embrionaria. El celo fue sincronizado en las receptoras utilizando PGF2α (Lutalyse®) o progesterona (PRO; Syncro-Mate-B). Un grupo de receptoras fue inyectado (im) con PGF los días 11 (25 mg), 0 (12,5 mg), y 1 (12,5 mg). Otro grupo fue tratado con PRO el dia -9 (implante auricular con 6 mg norgestomet e inyectadas (im) con 2 mL con 3 mg de norgestomet y 5 mg de valerato de estradiol), el implante fue removido el día 0. Los embriones fueron transferidos al azar a las receptoras en sincronía +24 a -24 h con embriones de 7-d de edad. Ambos métodos de sincronización (PGF vs. PRO) fueron similares en la inducción del celo (89,8 vs. 86,7 por ciento). El porcentaje de celos inducidos fue similar en Brahman (91,0 por ciento) y Angus (85,6 por ciento). Las receptoras tratadas con PGF presentaron un intervalo al celo más largo (P = 0,001) que aquellas tratadas con PRO (77,4 vs. 60,1 h). El intervalo al estro post-tratamiento fue más corto en la vacas Angus expresaron celo más temprano (P = 0,043) que las Brahman (65,4 vs. 72,2 h). La tasa de preñez no fue afectada por la raza (Angus 49 por ciento 35/72 y Brahman 54 por ciento 38/70; P = 0,97) ni por el tratamiento de sincronización (PRO 49 por ciento 35/72 y PGF 54 por ciento 38/70; P = 0,23). Las tasas de preñez en receptoras de sincronización cercana (0 h) fue del 60,0 por ciento, entre +12 y -12 h fue del 56,0 por ciento, y entre +24 y -24 h fue del 51,5 por ciento. Las receptoras de cuatro años de edad presentaron tasas de preñez menores (34,8 por ciento) que las de cinco (60,9 por ciento) o las de 6 o más años de edad (70,0 por ciento). Los resultados indican que cuando la segunda aplicación de PGF se administra dividida en dos dosis, es tan efectiva para sincronizar...

Eficácia da dose reduzida de gonadorelina e diferentes prostaglandinas no protocolo ovsynch em vacas holandesas / Efficacy of decreasing dose of gonadorelin and comparison between different prostaglandins on ovsynch protocol in lactating Holstein cows

Avaliou-se a eficácia da dose de 0,10 ou de 0,25mg de gonadorelina nas taxas de ovulação e de sincronização no protocolo Ovsynch e de 150mcg de D-cloprostenol ou 0,530mg de cloprostenol sódico na regressão do corpo lúteo (CL) de sete dias e de todos os CL. Foram utilizadas 136 vacas lactantes da raça Holandesa, com média de produção de leite de 23,75,8kg/dia, com 138,4±72,0 dias em lactação. As vacas foram distribuídas aleatoriamente em quatro grupos, de acordo com a dose de gonadorelina e o tipo da prostaglandina. As taxas de ovulação e de sincronização foram de 52,9 por cento e 80,9 por cento para 0,10mg de gonadorelina e de 57,4 por cento e 80,9 por cento para 0,25mg de gonadorelina, respectivamente. A taxa de regressão do CL de sete dias foi de 97,1 por cento para o D-cloprostenol e de 97,5 por cento para o cloprostenol sódico. A taxa de prenhez não foi influenciada pelos tratamentos, mas foi influenciada pela taxa de ovulação à primeira aplicação de gonadorelina, 16,0 por cento vs. 6,6 por cento para as vacas que ovularam e não ovularam, respectivamente. Conclui-se que 0,10mg de gonadorelina foi eficiente e ambas prostaglandinas podem ser usadas em protocolos de sincronização da ovulação.

The efficacy of 150mcg D-cloprostenol or 0.530mg cloprostenol sodium on the 7- day corpus luteum (CL) regression rate, and of two doses of gonadorelin, 0.10mg vs. 0.25mg, on the ovulation and synchronization rates during the Ovsynch protocol were evaluated. Lactating Holstein cows (n=136), producing 23.75.8kg milk/d and 138.4±72.0 days in milk were randomly assigned to four groups, according to dose of gonadorelin and prostaglandin type. No effect of the products and doses tested on conception rate were observed. The ovulation and synchronization rates using 0.10mg or 0.25mg of gonadorelin were 52.9 percent and 80.9 percent vs. 57.4 percent and 80.9 percent, respectively. The CL regression rates in cows ovulating after the first GnRH using D-cloprostenol or cloprostenol sodium were 97.1 percent and 97.5 percent, respectively. The pregnancy rate was not affected by treatment, but by ovulation rate after the first injection of GnRH. The pregnancy rates were 16.0 percent and 6.6 percent for ovulated and non ovulated cows, respectively. The results suggest that 0.10mg dose of gonadorelin was efficient and both prostaglandins can be used in synchronization of ovulation protocols.

Efecto de la sincronización del estro con prostaglandina-f2a vs CIDR + 500 UI de eCG en ovejas bergamacia durante el inicio de la fase luteal / Effect of estrus synchronization with prostaglandins-F2a vs CIRD + 500 IU of eCG in bergamacia ewes during early luteal phase

Con el objeto de evaluar los efectos de la sincronización del estro con PGF2a vs CIDR + 500 UI de eCG, sobre el tiempo de presentación de estro, ovulación y las concentraciones plasmáticas de hormonas esteroidales durante el inicio de la fase luteal en ovejas, se seleccionaron 14 hembras Bergamacia distribuidas en dos grupos: el Grupo uno (Control), sometido a dos aplicaciones de prostaglandina-F2a (PG), con un intervalo de nueve días, y el Grupo dos, tratado con el dispositivo intravaginal impregnado con progesterona (CIDR) durante 12 días y 500 UI de eCG. La presentación del estro fue de 100%. Entretanto, el intervalo estro-ovulación fue de 36,0 ± 0,72 horas en el grupo dos. El grupo control tratado con PG presentó un intervalo estro-ovulación de 53,42 ± 3,0 horas (P<0,001). Hubo diferencia significativa (P<0,01) en las concentraciones plasmáticas de P4 entre los tratamientos. Los animales del Grupo dos presentaron aumentos significativos (P < 0,01) en las concentraciones plasmáticas de P4, desde el sexto hasta el décimo día después de la ovulación, comparados con las concentraciones de los animales control. Del mismo modo, también se constató una diferencia significativa en la interacción tratamiento y día (P< 0,05). Las concentraciones de E2 en el plasma sanguíneo fueron estadísticamente diferentes (P< 0,001) entre el grupo control y el grupo sincronizado con el CIDR + eCG. Fueron observadas diferencias significativas (P< 0,001) en las concentraciones plasmáticas de E2 entre los tratamientos después del día ovulatorio (día cero), siendo además constatada diferencia significativa entre los días (P< 0,001) y en la interacción tratamiento y día (P< 0,05). Se puede concluir que, la sincronización del estro en hembras Bergamacia, utilizando el CIDR + eCG disminuyó el intervalo estro-ovulación y provocó aumento de las hormonas esteroidales en plasma.

An experiment was conducted to investigate the effects of estrous synchronization with PGF2a vs cidr + 500 IU of eCG on interval to estrus, ovulation and steroidal hormones plasma concentrations during early luteal phase in sheep. Fourteen ewes were treated, distributed in two groups: Group 1 (Control), synchronized with two injections of prostaglandin-F2a (PG), given 9 days apart, and Group 2, was treated with CIDR for 12 days and 500 IU of eCG. Estrous presentation was 100%. The interval estrus-ovulation was 36.0 ± 0.72 h in Group two. The control group presented an interval estrus-ovulation of 53.42 ± 3.0 hours (P<0.001).There were significant difference (P < 0.01) in P4 plasma concentrations among treatments. In Group 2, the animals showed significatives increases (P < 0.01) in P4 plasma concentrations during day 6 until day 10 when compared with control animals. It was observed significative difference at interaction treatment by day (P < 0.05). E2 plasma concentrations were statistically differents (P < 0.001) among control group and synchronized group with CIDR + eCG. There were significant difference (P < 0.001) in E2 plasma concentrations among treatments after ovulation day (Day 0) with significative difference among days (P < 0.001) and interaction treatment x day (P < 0.05). These results indicated that estrous synchronization in Bergamacia females, using CIDR + eCG diminished interval estrus-ovulation and elicit higher levels of steroidal hormones in plasma.

Efeitos de diferentes fontes de energia sobre taxa ovulatória, fertilidade e sobrevivência embrionária em marrãs cíclicas / Effects of different energy sources on ovulation rate, pregnancy rate and embryo survival in cyclic gilts

Cinqüenta e quatro marrãs cíclicas, uniformizadas quanto à linhagem, família, ganho de peso, espessura de toucinho, peso, precocidade sexual, número de cios e escore clínico, foram alocadas em dois grupos experimentais com dietas isocalóricas, isoprotéicas e isolisínicas. Duas fontes de energia foram testadas: amido de milho (T1) e óleo de soja (T2). Sincronizou-se o segundo estro com allyl-trenbolone, para inseminação no terceiro estro. Foi realizada cateterização não cirúrgica em 21 marrãs, submetidas a dois ciclos de coleta para dosagem de glicose e insulina, aos 14 e 21 dias do ciclo. Todas as marrãs foram abatidas aos 28,6 dias de gestação média, para análises biométricas do trato reprodutivo. Marrãs do T1 apresentaram maior taxa ovulatória em relação às do T2 (16,52 vs 14,70, P<0,01). Não houve diferença entre os tratamentos nas taxas de prenhez e sobrevivência embrionária. É possível alterar a eficiência reprodutiva por intermédio de manipulação dietética, mesmo em marrãs em estado anabólico. O uso do amido de milho na fase pré-cobertua melhorou a eficiência reprodutiva dos animais avaliados.

Fifty four cyclic gilts were randomly selected and uniformized according to genetic background, litter of origin, weight gain, backfat, number of cycles and clinical score. Gilts were alloted to one of two groups fed isocaloric, isoproteic and isolysinic diets. Two energy sources were tested: corn starch (T1) and soybean oil (T2). Second estrus was synchronized with oral allyl-trenbolone, so that insemination was carried out at third estrus. Indweeling catheters were implanted by non-surgical technic in 21 gilts, which were submitted to consecutive blood samplings for glicose and insulin determination. Timing of ovulation was estimated by means of ultrasonography. All gilts were slaughtered at an average gestation lenght of 28.6 days. Starch-fed gilts (T1) showed higher ovulation rates than T2 gilts (16.52 vs 14.70; P<0.01). There was no effect of treatments on pregnancy rate and embryo survival. Results indicate it is possible to manipulate reproductive efficiency through diet even in anabolic experimental models like cyclic gilts. Feeding starch as main energy source during pre-mating flushing phase improved reproductive efficiency of cyclic gilts.

Evaluación de cuatro protocolos de sincronización para inseminación a tiempo fijo en vacas Bos indicus lactantes / Evaluation of four synchronization protocols for fixed-time artificial insemination in Bos indicus lactating cows

El objetivo del estudio fue evaluar cuatro protocolos de sincronización para inseminación artificial a tiempo fijo (IATF) en vacas Bos indicus lactantes. Se seleccionaron 120 vacas Brahman entre 45 y 120 días postparto y fueron ubicadas aleatoriamente en uno de cuatro tratamientos. El tratamiento Crestar consistió en un implante auricular de norgestomet y una inyección de norgestomet y valerato de estradiol, el día 9 se retiró el implante y se aplicó eCG; la IATF se realizó 48-52 horas después. El tratamiento GPG consistió en una inyección de gonadorelina, el día 7 una inyección de D-cloprostenol y el día 9 una segunda inyección de gonadorelina e IATF 18-22 horas después. El tratamiento GPE fue similar al tratamiento GPG, excepto que la segunda dosis de GnRH fue reemplazada por benzoato de estradiol (BE) el día 8 e IATF 30-32 horas después. El tratamiento CIDR-B consistió en la aplicación del dispositivo intravaginal más una inyección de BE y otra de progesterona, 7 días después se retiró el dispositivo y se aplicó D-cloprostenol, el día 8 una inyección de BE y la IATF 30-32 horas después. El diagnostico de preñez fue determinado mediante ultrasonografia transrectal 35 días después de la IATF. El tratamiento Crestar tuvo una tasa de preñez superior (P<0,01) a los demás tratamientos (55,7 por ciento versus 19,4 por ciento, 22,5 por ciento y 21,8 por ciento, respectivamente). Los resultados del presente estudio indican que es posible obtener tasas de preñez aceptables con la IATF en vacas B. indicus lactantes y que los tratamientos con dispositivos de liberación de progesterona más eCG pueden mejorar el desempeño reproductivo de las vacas.

The objective of this study was the evaluation of four synchronization protocols for fixed-time artificial insemination (FTAI) in Bos indicus lactancting cows. Brahman cows (n=120) between 60 and 120 days postpartum were randomly assigned to one of four syncronization treatments. The Crestar treatment consisted of the aplication of ear implant of norgestomet and an injection of norgestomet and estradiol valerate, on day 9 the implant was removed and the cows received an eCG injection; the FTAI was performed 48-52 hours later. In the GPG treatment the cows received an injection of gonadorelina on day 0, an injection of D-cloprostenol on day 7, a second injection of gonadorelina on day 8 and FTAI 18-22 hours later. The GPE treatment was similar to GPG, but the second dose of gonadorelina was replaced by an estradiol benzoate (EB) injection and the FTAI was performed 30-32 hours later. The CIDR-B treatment consisted of the aplication of intravaginal device, an injection of EB and progesterone, the device was removed 7 days later, at the same time the cows received an injection of D-cloprostenol, on day 8 the cows received an injection of EB and FTAI 30-32 hours later. Pregnancy was diagnosed by transrectal ultrasonography 35 days after TAI. Crestar treatment has a higher pregnancy rate (P<0,01) than the other treatments (55.7 vs 19.4, 22.5 y 21.8% respectively). The findings of this experiment show that is possible to get acceptable pregnancy rates with FTAI in B. indicus lactating cows and progesterone releasing devices plus eCG treatments should improve reproductive performance of cows.

Efeito do intervalo entre recrutamentos foliculares na superovulação de vacas da raça Holandesa não-lactantes / Effect of interval between follicular recruitments on superovulatory response in non-lactanting Holstein cows

Vacas da raça Holandesa não-lactantes, distribuídas em dois grupos, foram sincronizadas com o protocolo Ovsynch modificado. No dia sete (dia 0 = dia do segundo GnRH), o grupo 7 (G-7; n=19) recebeu CIDR usado previamente por cinco dias e 100mcg de GnRH, e o grupo 14 (G-14; n=21), CIDR e 25mg de PGF2alfa. No dia 14 foi aspirado o folículo dominante (FD), trocado o CIDR usado por um novo e foram aplicados 25mg de PGF2alfa. Iniciou-se o tratamento com FSH 36h depois, removeu-se o CIDR com o sétimo FSH e aplicou-se GnRH 36h depois. As inseminações foram feitas 12 e 24h depois. Recuperaram-se os embriões sete dias depois da inseminação artificial. O diâmetro do FD no G-7 foi 13,1±0,57mm no dia sete e 11,2±0,57mm no dia 14. O diâmetro FD persistente no G-14 aumentou de 12,6±0,55mm no dia sete para 16,4±0,55mm no dia 14 (P<0,001). O número de folículos >8mm, 48h após o início do tratamento com FSH, foi maior (P<0,05) no G-7 (15,6±0,05) que no G-14 (12,5±0,05). Não foi detectado efeito de tratamento sobre o número de corpos lúteos e de embriões. O menor intervalo entre recrutamentos foliculares aumentou o número de folículos recrutados, porém não alterou a quantidade e a qualidade dos embriões produzidos

Dry Holstein cows were synchronized by a modified Ovsynch protocol. On day 7 (day 0 = day of the second GnRH), group 7 (G-7; n=19) received an used CIDR and GnRH (100'g). Group 14 (G-14; n=21) received an used CIDR and PGF2alpha (25mg). On day 14, the dominant follicle (DF) was removed, by aspiration; the used CIDR was changed for a new CIDR plus PGF2alpha. Thirty-six hours later, the FSH treatment was initiated. On the seventh injection of FSH, the CIDR was removed. Thirty-six hours after, the cows received GnRH. Donors were inseminated 12 and 24h after the GnRH injection. Embryos were recovered seven days after AI. The DF diameter in G-7 was 13.1mm on day 7 and 11.2mm on day 14. The persistent DF in the G-14 increased from 12.6mm on day 7 to 16.4mm on day 14 (P<0.001). The number of follicles >8mm, 48h after the beginning of the FSH treatment, was 15.6 in G-7 and 12.5 in G-14 (P<0.05). No treatment effects were detected on number of corpus luteum and embryos. The shorter interval between follicular recruitment the higher the number of recruited follicles, but it did not have effects on the number or quality of embryos produced

Concentração sérica de progesterona em bezerras da raça nelore e mestiças tratadas com progesterona em veículo de liberação lenta / Serum progesterone concentration in Nelore and crossbreed heifers treated with long-acting progesterone

Avaliaram-se a eliminação da progesterona em veículo de liberação lenta (P4LA) em animais zebus e mestiços e sua potencial aplicabilidade em programas de sincronização de estro, utilizando-se 60 bezerras, 30 da raça Nelore e 30 mestiças (Gir x Holandês), entre 120 e 150 dias de idade e peso médio de 150kg. Em cada grupo experimental as bezerras foram divididas em três subgrupos (G) de 10 animais, sendo GI = controle (tratado com 5ml de solução fisiológica por via intramuscular); GII = tratado com 450mg P4LA (3ml IM); e GIII = tratado com 750mg P4LA (5ml IM). Amostras de sangue foram coletadas no dia zero, 7 e 13 (D0, D7 e D13) e procedeu-se à análise hormonal por radioimunoensaio de fase sólida. A progesterona de ação prolongada (P4LA), administrada por via intramuscular, manteve-se por 13 dias na corrente sangüínea em concentrações superiores a 1ng/ml. As doses de 450mg e 750mg de P4LA não ocasionaram efeitos adversos sistêmicos clinicamente perceptíveis, e o metabolismo da P4LA foi mais lento nas bezerras Nelore, cuja concentração de progesterona foi maior na corrente sangüínea do que nas bezerras mestiças.

The clearance of long-acting progesterone in microspheres (P4LA) in zebu animals and its potential for use in estrus synchronization were evaluated using 30 Nelore and 30 crossbreed (Holstein x zebu) heifers, with aging between 120 to 150-day-old and weighting 150kg in average. For each breed the animals were divided into three groups of ten animals each, GI= control group treated with saline; GII= treated with 450mg of P4LA; and GIII= treated with 750mg of long-acting progesterone (P4LA). Blood samples were colleted on days 0, 7 and 13 and analysed for progesterone using radioimmunoassay in solid phase. The serum concentration of progesterone was different on days 0, 7 and 13 in relation to the dose of P4LA given. All treated animals presented basal values for progesterone on day 0, increased on day 7 and decreased on day 13, but with values over 1ng/ml. The results show that the drug was absorbed rapidly after its administration and remained in satisfactory concentration in the circulation until day 13. The average total concentration of serum progesterone was higher in Nelore heifers when compared to the concentration in crossbreed ones, indicating difference in the metabolism of the drug between the two genetic groups.

Uso do protocolo Crestar® em tratamentos utilizando benzoato de estradiol, PGF2alfa, PMSG e GnRH para controle do ciclo estral e ovulação em vacas de corte / Sincronization of ovulation utilizing the crestar® protocol associated with estradiol benzoate, PGF2alfa, PMSG and GnRH in beef cows

O objetivo foi avaliar a eficiência do controle da ovulação utilizando os hormônios PMSG, GnRH, Benzoato de Estradiol e PGF2 α junto ao protocolo Crestar®, para IA em tempo fixo. Foram utilizadas 348 vacas, cruzadas Nelore (Bos taurus indicus) X Charolês (Bos taurus taurus), divididas em dois grupos: 179 vacas paridas com 90 a 120 dias pós-parto e 169 vacas solteiras. Estes animais foram submetidos a cinco tratamentos: todas as vacas receberam o protocolo Crestar® como agente sincronizador do crescimento folicular. (implante subcutâneo com 3mg de norgestomet e injeção 3mg de norgestomet + 5mg de valerato de estradiol - i.m.). Após a remoção do implante (10 dias), as vacas foram submetidas aos cinco tratamentos de controle da ovulação: T1 - (n=70): injeção de solução fisiológica 48h após a retirada do implante (D 12); T2 - (n=68): 0,75mg de benzoato de estradiol 24h após a retirada do implante (D 11); T3 - (n=70): aplicação de 150µg de PGF2 α, no dia da retirada (D 10) e 0,75mg de benzoato de estradiol 24h após a retirada do implante (D 11); T4 - (n=70): 500 UI de PMSG na retirada do implante (D 10) e T5 - (n=70): 500µg de GnRH 48h após a retirada dos implante (D 12). Todos os animais foram inseminados 54-56h após a retirada do implante. As taxas de prenhez foram analisadas estatisticamente por regressão logística. Não houve diferença entre os tratamentos (p>0,05) onde: 35,7, 31,4, 22,0, 37,0 e 42,8% para T1, T2, T3, T4 e T5 respectivamente.

The objective of this study was to evaluate the effects of ovulationusing PMSG, GnRH, Estradiol Benzoate and PGF2α in combination with Crestar® protocol and AI at fixed time. Three hundred forty eight multiparous cows, crossbreed Nelore (Bos taurus indicus) X Charolais (Bos taurus taurus) were divided in two groups: 179 suckling cows and 169 non-suckling cows. Those cows received a Crestar® protocol for follicular growth synchronization consisting of a subcutaneous implant with 3mg of norgestomet and 3mg of norgestomet plus 5mg of estradiol valerate injection (day of implant insert). The implant was removed after nine days. Cows were submitted to five treatments for pharmacological control of ovulation and were artificially inseminated at fixed time: T1 - (n=70): injection of physiological solution 48h after implant removal (D 12); T2 - (n=68): 0.75mg of estradiol benzoate 24h after implant removal (D 11); T3 - (n=70): 150µg of PGF2α at same day of implant removal (D 9) and 0.75mg of estradiol benzoate 24h after implant removal (D 11); T4 - (n=70): the cows received 500 UI of PMSG at implant removal (D 10) and T5 - (n=70): cows received 500µg of GnRH 48h after implant removal (D 12). Those cows were artificially inseminated 54-56h after implant removal. Pregnancy rate was analyzed by logistical regression program. There were no differences among treatments (P>0.05) 35.7, 31.4, 22.0, 37.0 and 42.8% for T1, T2, T3, T4 and T5, respectively.

Avaliação da liberação de lh e da concentração de progesterona em protocolos ovsynch e heatsynch em bubalinos (Bubalus bubalis) / Evaluation of lh surge and progesterone concentration in ovsynch and heatsynch protocols in buffalo (Bubalus bubalis)

O objetivo desse estudo foi avaliar a liberação de LH durante osprotocolos de sincronização da ovulação em bubalinos. Para tanto, quinze búfalas multíparas receberam 25 mg de Lecirelina no Dia 0, e150 mg de D-Cloprostenol no Dia 7. No Dia 8, foi aplicado benzoato de estradiol nas búfalas do Grupo 1 (0,5 mg, n = 5) e do Grupo 2 (1,0mg, n = 5). No Dia 9, os animais receberam 25mg de Lecirelina(Controle, n = 5). Para mensuração das concentrações plasmáticas de LH foram colhidas amostras de sangue da veia jugular no Dia 7, e em seguida em intervalos de 3 horas até completar 72 horas após a aplicação de PGF2a. Para avaliação da liberação de LH foram comparados os momentos de ocorrência do pico LH em relação à PGF2a, as durações, as amplitudes e a área sob o pico de pré-ovulatório de LH. As búfalasdos Grupos Controle, 1 e 2 apresentaram picos pré-ovulatórios deLH em 51 + 0,0 horas, 47,3 + 2,7 horas e 47,0 + 3,8 horas após a aplicação da PGF2a, respectivamente (P>0,05). A duração do pico deLH foi menor no Controle (7,8 + 1,5 horas) do que nos Grupos 1 e2 (10,5 + 1,5 horas vs. 10,8 + 2,4 horas, respectivamente; P < 0,05). Aamplitude média dos picos pré-ovulatórios de LH foram de 4,5 + 0,4ng/mL, 4,0 + 0,4 ng/mL e 4,3 + 0,8 ng/mL para os Grupos Controle,1 e 2, respectivamente (P > 0,05). A área sob o pico de LH no Controle(4,8 ± 0,7) foi menor do que as áreas dos Grupos 1 e 2 (8,8 ± 2,5 vs.8,7 ± 2,2, respectivamente; P < 0,05). Em resumo, a aplicação debenzoato de estradiol proporcionou maior duração e área do picopré-ovulatório de LH do que a administração de GnRH em protocolos Ovsynch em bubalinos.

The objective of this study was to evaluate the LH surge after lasthormonal injection of synchronization of ovulation protocols inbuffalo. Fifteen multiparous buffaloes received 25 mg of Lecirelin inDay 0, and 150 mg of D-Cloprostenol on Day 7. On Day 8, estradiolbenzoate was injected in Group 1 (0.5 mg, n = 5) and Group 2 (1.0mg, n = 5). On Day 9, five buffaloes received 25 mg of Lecirelin(Control). Blood samples were collected for measure the LHconcentrations on Day 7 and then every 3 hours until 72 hours afterthe PGF2a injection. For evaluation of LH surge were compared theinterval between PGF2a injection to LH surge, duration, amplitudeand area under the LH peak. The LH surge occurred 51.0 + 0.0 hours,47.3 + 2.7 hours and 47.0 + 3.8 hours after PGF2a injection for Control,Group 1 and Group 2, respectively (P > 0.05). The duration of LHpeak in Control (7.8 + 1.5 hours) was shorter than Groups 1 and 2(10.5 + 1.5 hours vs. 10.8 + 2.4 hours, respectively; P < 0.05). Theamplitudes of LH peak were 4.5 + 0.4 ng/mL, 4.0 + 0.4 ng/mL and4.3 + 0.8 ng/mL for Control, Group 1 and Group 2, respectively (P> 0.05). The area under LH peak for Control (4.8 ± 0.7) was smallerthan the areas of the Groups 1 and 2 (8.8 ± 2.5 vs. 8.7 2.2, respectively;P < 0.05). In summary, the estradiol benzoate injection providedhigher duration and area of LH peak than GnRH injection in Ovsynchprotocol in buffalo.

Fertilidade após a inseminação artificial intra-cervical ou laparoscópica intra-uterina de ovelhas utilizando diluidores à base de água de coco / Fertility following intracervical or intrauterine laparoscopic insemination of ewes using extenders based on coconut water

O objetivo deste estudo foi avaliar a taxa de prenhez em ovelhas utilizando diluidores à base de água de coco (in natura: ACN ou em pó: ACP-102®) após inseminação artificial intra-cervical (Experimento 1) ou laparoscópica intra-uterina (Experimento 2). Experimento 1. Sêmen de 4 carneiros Santa Inês foi coletado, diluído a 37°C (T1: ACN; T2: ACP-102®) e resfriado á 4°C. 56 ovelhas Santa Inês foram inseminadas cervicalmente após a sincronização, 31 utilizando o diluidor ACN e 25 o diluidor ACP-102®. Experimento 2. Sêmen de 5 carneiros adultos (Santa Inês: n=04; Dorper n=01) foi coletado e processado de acordo com o descrito. 64 ovelhas SRD foram inseminadas intra-uterina por laparoscopia após a sincronização do estro, 37 utilizando o diluidor ACN e 27 o diluidor ACP-102®. O diagnóstico de gestação foi realizado por ultra-sonografia. A taxa de prenhez entre os tratamentos, dentro de cada método de inseminação, entre cada diluidor para ambos métodos de inseminação foi analisada pelo teste do Qui-quadrado (p<0,05). A taxa de prenhez após IA intra-cervical (ACN: 25,8% vs. ACP-102®: 48%) e laparoscópica (ACN: 72,9% vs ACP-102®: 70,3%) não foi influenciada pelos diluidores. A taxa de prenhez foi influenciada pelo método de inseminação (intra-cervical: 35,7% vs.laparoscopia: 71,8%; p<0,05). Os diluidores a base de água de coco podem ser utilizados nas inseminações artificiais cervical ou laparoscopica em ovelhas do Nordeste do Brasil em condições de campo.

The objetive of this study was to evaluate pregnancy rates in ewes using extenders based on coconut water (in natura: NCW or in powder: PCW-102®) following intra - cervical (Trial 1) or intrauterine laparoscopic insemination (Trial2). Trial 1. Semen from 4 Santa Inês rams were collected, diluted at 32°C (T1: NCW; T2: PCW-102®) and cooled to 4°C. 56 adult Santa Inês ewes were inseminated intra-cervically following syncronization, 31 using the extender NCW and 25 the extender PCW-102®. Trial 2. Semen from 5 rams (Santa Inês: n=04; Dorper n=01) were collected and processed as described. 64 adult crossbred multiparous ewes were inseminated intrauterine by laparoscopy following oestrus syncronization. 37 ewes using the extender NCW and 27 the extender PCW-102®. The pregnancy diagnosis was performed by ultrasonography. The fertility rates between treatments for each method of insemination and within each extender for both methods were analysed by Chi-square test (p<0,05). The pregnancy rates following intra-cervical (NCW: 25.8% vs PCW-102®: 48%) and intrauterine (NCW: 72.9% vs 70.3%) inseminations were not influenced by the extenders. The pregnancy rate was influenced by insemination method (intra-cervical: 35.7% vs laparoscopy: 71.8%; p<0,05). The extenders based coconut water as basis can be used following cervical or intra-uterine laparoscopic inseminations in ewes from Northeast of Brazil under field conditions.

Indução da puberdade e sincronização do cio subsequente em leitoas pré-púberes utilizando gonadotrofinas exógenas / Induction of puberty and following estrus synchronization in prepubertal gilts using exogenous gonadotropins

A utilização de gonadotrofinas exógenas na indução da puberdade e sincronização do estro e ovulação é uma prática que influencia positivamente no manejo das marrãs, para a mais efetiva e econômica inclusão no plantel de matrizes em Sistemas Intensivos de Produção de Suínos. No entanto, poucos estudos têm sido desenvolvidos com a finalidade de avaliar o grau de sincronização do estro seguinte à indução da puberdade. Assim sendo, o objetivo do presente estudo foi, justamente, o de verificar a taxa de sincronização do estro subseqüente à puberdade induzida hormonalmente, e a comparação das características dos estros, induzido e subseqüente a este. Sessenta e seis leitoas pré-púberes tiveram a puberdade induzida através de combinação hormonal utilizando gonadotrofinas (eCG e LH) aos 145 dias de idade em média. Das 66 fêmeas que iniciaram o experimento, 35 (53,03%) apresentaram cio até sete dias após a aplicação do LH, e 40 (60,60%) das 66 fêmeas tiveram o estro subseqüente sincronizado, ou seja, até o 25° dia após a aplicação do LH. Vinte e seis (39,39%) fêmeas manifestaram o primeiro e segundo cios. As 26 (39,39%) leitoas que não manifestaram o 2° cio de maneira sincronizada foram abatidas, sendo que 15 apresentaram corpos lúteos e/ou corpos albicans e 11 não apresentaram as estruturas mencionadas anteriormente, sendo consideradas imaturas. Não houve diferença significativa da duração do cio na puberdade com o cio subseqüente (P>0,05), tampouco da dispersão dos mesmos.

The utilization of exogenous gonadotropins in the puberty induction and estrus/ovulation synchronization is a management that influence positive1y gilts handle, to the better effectiveness and economic inclusion of gilts in the Intensive Swine Production System herd. However, few researches have been deve10ped to evaluate the subsequent estrus synchronization after puberty induction. Thus, the aim of this study was to verify the subsequent estrus synchronization after the hormonal puberty induction and to evaluate the inducted and the following one estrus characteristics. Sixty six prepubertal gilts had their puberty inducted by a hormonal combination using gonadotropins (eCG and LH) at 145 days old, in average. A total of 35 (52,03%) of the 66 initially gilts showed estrus up to seven days after LH administration, and 40 (60,60%) had the subsequent estrus synchronized (synchronization was defined as the gilts which showed estrus up to day 25 after LH administration). Twenty six gilts (39,39%) showed the first and second estrus. The 26 (39,39%) remaining gilts that didn't show the second estrus synchronized were slaughtered, which 15 presented corpus albicans and/or corpora lutea and 11 were considered immature because they didn't present any structure mentioned above in the ovaries. There was no significant difference between the puberty estrus duration and the subsequent estrus duration (P>0,05), neither their dispersion.

Effect of insemination-to-induced ovulation interval on fertilization rate, embryo viability and number of accessory sperms in SOWS

The ideal interval between AI and ovulation (OV) is not well determined yet, varying from 12 to 28 h before up to 4 h after ovulation. Utilization of gonadotrophins to synchronize ovulation would allow the pre-determination of the groups' size, according to the AI-OV intervals, and would contribute to determine a secure interval between AI-Ov. 120 sows received 7.5 mg IM of Luprostiol, between days 12 and 17 of the estrous cycle, 600 lU of eCG 1M 24 h after prostaglandin and 5.0 mg of LH 1M 72 h after eCG injection. The moment of ovulation was diagnosed by transrectal ultrasonography at intervals of 6 h. There were 5 treatments according to IA-OV interval: T1- 48 to 36 h before OV; T2- 36 to 24 h before OV; T3- 24 to 12 h before OV; T4- 12 to 0h before OV and T5-0 to 12 h after Ov. Sows were slaughtered 96. 7±11.37 h after Ov. Recovery rate (RR), number of corpora lutea (NC), total number of structures (ST), fertilization rate (FR), embryo viability (EV) and number of accessory sperm (AS) were analyzed. The synchronization protocol showed an homogenous distribution of the animaIs among treatments (LH-OV interval 39.22±7.6h), and it didn't influenced the results. FR and EV results suggest that 36 h is the time of sperm viability in sow genital tract. There was a strong decline of AS between T3 and T4.

O intervalo ideal entre a AI e ovulação (OV) não está bem determinado ainda, variando entre 12 a 28 h antes até 4 h depois da ovulação. A utilização de gonadotrofinas para sincronizar ovulação permitiria a pré-determinação do tamanho dos grupos, de acordo com os intervalos IA-OV, e possibilitaria determinar um intervalo seguro entre IA-OV 120 porcas receberam 7.5 mg IM de Luprostiol, entre os dias 12 e 17 do ciclo estral, 600 lU de eCG 1M 24 h após o Luprostiol e 5.0 mg de LH 1M 72 h após a injeção de eCG. O momento de ovulação foi diagnosticado pela ultra-sonografia trans-retal a intervalos de 6 h. Definiu-se 5 tratamentos de acordo com o intervalo IA-OV: T1 - 48 a 36 h antes da OV; T2 - 36 a 24 h antes da OV; T3 - 24 a 12 h antes da OV; T4 -12 a 0 h antes da OV e T5 - 0 a 12 h após a OV. O abate ocorreu 96.7±11.37 h após a OV. A taxa de recuperação (RR), número de lutea de corpos (NC), número total de estruturas (ST), taxa de fecundação (FR), viabilidade embrionária (EV) e número de espermatozóides acessórios (AS) foram analisados. O protocolo de sincronização mostrou uma distribuição homogênea dos animais entre os tratamentos (intervalo LH-OV de 39.22±7.6h), e não influenciou os resultados. A FR e os resultados de EV sugerem que 36 h seja o tempo de viabilidade do espermatozóide trato genital da porca. Houve um forte declínio do AS entre T3 e T4.

Pregnancy rate in Egyptian buffaloes after synchronization of estrus with PGF2alpha or ovulation by ovsynch program [with 2 tables and 2 figures]

Postpartum in buffaloes plays a great role in delay of the cyclicity in buffaloes that seemed to be anestrous. So, the use of hormones to induct estrus or induct ovulation has an importance to increase pregnancy rate. The present study was designed to evaluate the efficacy of the two methods in inducing high pregnancy rate. The experimental animals [48 lactating buffaloes] were divided into three groups: PGF2alpha group 1[n=16]; Ovsynch group 2 [n=16] and control group [n=16]. Pregnancy rate was significantly [p<0.001]increased in group 2 than group 1 and control group due to higher level of progesterone and more accurate synchronization for ovulation., It is more suitable to use the regimen of ovsynch in postpartum buffaloes especially in multiparous females with good condition score

Utilização de gonadotrofina coriônica humana e cipionato de estradiol associado ao dispositivo de liberação controlada de drogas para sincronização de ovulação em cabras da raça Saanen / Use of human chorionic gonadodrophin and estradiol cipionate plus controlled internal drug release for synchronization of ovulation in Saanen goats

Dezesseis cabras nulíparas da raça Saanen foram distribuídas em dois grupos de tratamentos (T1 e T2) para sincronização da ovulação. Inicialmente, ambos os tratamento consistiram na aplicação concomitante do dispositivo de liberação controlada de drogas (CIDR-G®), de 5mg de dinoprost e de 1mg de cipionato de estradiol (CE) (dia 0). No quarto dia aplicaram-se 250UI de eCG e no quinto dia retirou-se o CIDR-G®. As cabras do T1 (n=8) receberam 1mg de CE 24 horas depois da retirada do CIDR-G® e as do T2 (n=8) receberam 250UI de hCG 30 horas após. Sete cabras do T1 e oito do T2 entraram em estro depois da retirada do CIDR-G®. Cabras que receberam hCG permaneceram em estro por 42,0± 6,9 horas e as que receberam CE por 45,0± 5,5 horas (P>0,05). As características ovulatórias não foram influenciadas pelos tratamentos. O intervalo da retirada do CIDR-G® à ovulação para ambos os protocolos de sincronização da ovulação não diferiu (P>0,05) entre tratamentos. As ovulações promovidas pelo CE ocorreram em menor intervalo de tempo.